controls e coli Search Results


99
ATCC escherichia coli atcc 25922
Escherichia Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher ice cold bsa pbs
Ice Cold Bsa Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Cole-Parmer x sspe
X Sspe, supplied by Cole-Parmer, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs high efficiency neb 5 alpha competent escherichia coli
High Efficiency Neb 5 Alpha Competent Escherichia Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
ATCC e coli atcc 25298
E Coli Atcc 25298, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ZeptoMetrix corporation zeptometrix respiratory control panels
Zeptometrix Respiratory Control Panels, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Addgene inc escherichia coli bl21 de3 strain
Figure 1. DET1- and COP1-associated proteins. (A) Schematic representation of proteins found to associate with DET1 and COP1 in TAP assays. Color code represents the maximum number of peptides for each represented protein found in a TAP assay as detailed in Supplemental Table 1. DET1 and COP1 proteins were expressed in Arabidopsis cell cultures. Five independent TAP experiments were performed for DET1 and two for COP1 (Supplemental Table 2). (B) MBP-COP1 and MBP-HY5 recombinant proteins expressed in E. coli pulled-down MYC-DET1 from 7-day-old Arabidopsis seedlings. MBP re- combinant protein was used as a control. Anti-MYC and anti-MBP antibodies were used for the immunoblots. (C and D) F€orster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET–FLIM) analysis of the interaction between COP1 or HY5 fused to GFP and DET1 (C), and HY5 or COP1 fused to RFP (D). Box plots show the distribution of 5–9 measurements ±SD. (E) FRET–FLIM analysis of the interaction between GFP-COP1 and RFP-HY5 upon cluc-DET1 co-expression. Box plots show the distribution of 10 measurements ±SD. All FRET assays were performed following transient expression in N. benthamiana leaves. Free RFP was used as negative control. FE, FRET efficiency. Asterisks indicate statistically significant differences according to Student’s t-test (****p < 0.0001; ***p < 0.001; *p < 0.01). For all FRET experiments three independent biological replicates were performed, and results from one replicate are shown.
Escherichia Coli Bl21 De3 Strain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Santa Cruz Biotechnology rabbit polyclonal anti fgf10 antibody
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Rabbit Polyclonal Anti Fgf10 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
ATCC e coli 348 69 eae
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
E Coli 348 69 Eae, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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98
New England Biolabs competent escherichia coli bacteria
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Competent Escherichia Coli Bacteria, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
ATCC e coli atcc 7624 strain
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
E Coli Atcc 7624 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Cell Signaling Technology Inc phospho smad3 ser423 425 rabbit mab
Fig. 2. Effect of cyadox on the expressions of STATs and Smads mRNA in swine hepatocyte. AG490 (Fig. 2A-C) or SB431542 (Fig. 2D-G) were applied in 2 μM cyadox- treated swine hepatocyte. The expression of STAT1, STAT3, STAT5B, Smad1, Smad2, <t>Smad3</t> and Smad4 were detected by qRT-PCR. The results were presented as Mean ± SD. (n = 6). Significant differences were indicated by * p < 0.05, ** p < 0.01, versus control and # p < 0.05, ## p < 0.01, versus cyadox.
Phospho Smad3 Ser423 425 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. DET1- and COP1-associated proteins. (A) Schematic representation of proteins found to associate with DET1 and COP1 in TAP assays. Color code represents the maximum number of peptides for each represented protein found in a TAP assay as detailed in Supplemental Table 1. DET1 and COP1 proteins were expressed in Arabidopsis cell cultures. Five independent TAP experiments were performed for DET1 and two for COP1 (Supplemental Table 2). (B) MBP-COP1 and MBP-HY5 recombinant proteins expressed in E. coli pulled-down MYC-DET1 from 7-day-old Arabidopsis seedlings. MBP re- combinant protein was used as a control. Anti-MYC and anti-MBP antibodies were used for the immunoblots. (C and D) F€orster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET–FLIM) analysis of the interaction between COP1 or HY5 fused to GFP and DET1 (C), and HY5 or COP1 fused to RFP (D). Box plots show the distribution of 5–9 measurements ±SD. (E) FRET–FLIM analysis of the interaction between GFP-COP1 and RFP-HY5 upon cluc-DET1 co-expression. Box plots show the distribution of 10 measurements ±SD. All FRET assays were performed following transient expression in N. benthamiana leaves. Free RFP was used as negative control. FE, FRET efficiency. Asterisks indicate statistically significant differences according to Student’s t-test (****p < 0.0001; ***p < 0.001; *p < 0.01). For all FRET experiments three independent biological replicates were performed, and results from one replicate are shown.

Journal: Molecular plant

Article Title: DET1-mediated COP1 regulation avoids HY5 activity over second-site gene targets to tune plant photomorphogenesis.

doi: 10.1016/j.molp.2021.03.009

Figure Lengend Snippet: Figure 1. DET1- and COP1-associated proteins. (A) Schematic representation of proteins found to associate with DET1 and COP1 in TAP assays. Color code represents the maximum number of peptides for each represented protein found in a TAP assay as detailed in Supplemental Table 1. DET1 and COP1 proteins were expressed in Arabidopsis cell cultures. Five independent TAP experiments were performed for DET1 and two for COP1 (Supplemental Table 2). (B) MBP-COP1 and MBP-HY5 recombinant proteins expressed in E. coli pulled-down MYC-DET1 from 7-day-old Arabidopsis seedlings. MBP re- combinant protein was used as a control. Anti-MYC and anti-MBP antibodies were used for the immunoblots. (C and D) F€orster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET–FLIM) analysis of the interaction between COP1 or HY5 fused to GFP and DET1 (C), and HY5 or COP1 fused to RFP (D). Box plots show the distribution of 5–9 measurements ±SD. (E) FRET–FLIM analysis of the interaction between GFP-COP1 and RFP-HY5 upon cluc-DET1 co-expression. Box plots show the distribution of 10 measurements ±SD. All FRET assays were performed following transient expression in N. benthamiana leaves. Free RFP was used as negative control. FE, FRET efficiency. Asterisks indicate statistically significant differences according to Student’s t-test (****p < 0.0001; ***p < 0.001; *p < 0.01). For all FRET experiments three independent biological replicates were performed, and results from one replicate are shown.

Article Snippet: Pull-down assays MBP recombinant protein fusions were expressed in the Escherichia coli BL21 (DE3) strain carrying the corresponding coding sequence cloned into the pKM596 plasmid, a gift from David Waugh (Addgene plasmid #8837).

Techniques: Recombinant, Control, Western Blot, Förster Resonance Energy Transfer, Imaging, Microscopy, Expressing, Negative Control

Figure 5. Fgf10-induced IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.

Journal: Cell reports

Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.

doi: 10.1016/j.celrep.2021.108772

Figure Lengend Snippet: Figure 5. Fgf10-induced IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1

Techniques:

Figure 6. Fgf10 is required for maintaining papillary structure defined as IPNB (A) Experimental design of the withdrawal of Dox and/or TAA administration to iFGF10 mice: (1) Dox-only (Dox+) administration and (2) TAA+Dox+ administration followed by the withdrawal (OFF) (N = 5 each). ‘‘IPNB establishment time’’ and ‘‘endpoint time’’ are indicated.

Journal: Cell reports

Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.

doi: 10.1016/j.celrep.2021.108772

Figure Lengend Snippet: Figure 6. Fgf10 is required for maintaining papillary structure defined as IPNB (A) Experimental design of the withdrawal of Dox and/or TAA administration to iFGF10 mice: (1) Dox-only (Dox+) administration and (2) TAA+Dox+ administration followed by the withdrawal (OFF) (N = 5 each). ‘‘IPNB establishment time’’ and ‘‘endpoint time’’ are indicated.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1

Techniques:

Fig. 2. Effect of cyadox on the expressions of STATs and Smads mRNA in swine hepatocyte. AG490 (Fig. 2A-C) or SB431542 (Fig. 2D-G) were applied in 2 μM cyadox- treated swine hepatocyte. The expression of STAT1, STAT3, STAT5B, Smad1, Smad2, Smad3 and Smad4 were detected by qRT-PCR. The results were presented as Mean ± SD. (n = 6). Significant differences were indicated by * p < 0.05, ** p < 0.01, versus control and # p < 0.05, ## p < 0.01, versus cyadox.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Signaling pathways involved in the expression of SZNF and the target genes binding with SZNF related to cyadox.

doi: 10.1016/j.biopha.2018.09.141

Figure Lengend Snippet: Fig. 2. Effect of cyadox on the expressions of STATs and Smads mRNA in swine hepatocyte. AG490 (Fig. 2A-C) or SB431542 (Fig. 2D-G) were applied in 2 μM cyadox- treated swine hepatocyte. The expression of STAT1, STAT3, STAT5B, Smad1, Smad2, Smad3 and Smad4 were detected by qRT-PCR. The results were presented as Mean ± SD. (n = 6). Significant differences were indicated by * p < 0.05, ** p < 0.01, versus control and # p < 0.05, ## p < 0.01, versus cyadox.

Article Snippet: Phospho-Akt (Ser473) Antibody, Phospho-p38 MAPK (Thr180/Tyr182) Antibody, Phospho-Smad3 (Ser423/425) Rabbit mAb, Phospho-Stat1 (Tyr701) Rabbit mAb, Phospho-NF-κB p65 (Ser536) Rabbit mAb and Phospho-SAPK/JNK (Thr183/Tyr185) Rabbit mAb were purchased from Cell Signaling Technology (Beverly, Massachusetts, USA), Inc. Phospho-Stat5B Rabbit Antibody was obtained from Abcam CO. (Cambridge, UK).

Techniques: Expressing, Quantitative RT-PCR, Control

Fig. 3. Cyadox regulated the phosphorylation of signal factors in swine hepatocyte. A-D: The phosphorylation level of Smad3, STAT1, p38 and Akt in the presence of 2 μM cyadox and/or inhibitors of TGF-β, JAK/STAT, p38 MAPK and PI3K/Akt in swine hepatocyte were detected. E: The phosphorylation level of Smad3, STAT1, p38 and Akt after cyadox treatment at 0.5, 1, and 2 h. Significant difference was indicated by * p < 0.05, ** p < 0.01, versus control. # p < 0.05, ## p < 0.01, versus cyadox.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Signaling pathways involved in the expression of SZNF and the target genes binding with SZNF related to cyadox.

doi: 10.1016/j.biopha.2018.09.141

Figure Lengend Snippet: Fig. 3. Cyadox regulated the phosphorylation of signal factors in swine hepatocyte. A-D: The phosphorylation level of Smad3, STAT1, p38 and Akt in the presence of 2 μM cyadox and/or inhibitors of TGF-β, JAK/STAT, p38 MAPK and PI3K/Akt in swine hepatocyte were detected. E: The phosphorylation level of Smad3, STAT1, p38 and Akt after cyadox treatment at 0.5, 1, and 2 h. Significant difference was indicated by * p < 0.05, ** p < 0.01, versus control. # p < 0.05, ## p < 0.01, versus cyadox.

Article Snippet: Phospho-Akt (Ser473) Antibody, Phospho-p38 MAPK (Thr180/Tyr182) Antibody, Phospho-Smad3 (Ser423/425) Rabbit mAb, Phospho-Stat1 (Tyr701) Rabbit mAb, Phospho-NF-κB p65 (Ser536) Rabbit mAb and Phospho-SAPK/JNK (Thr183/Tyr185) Rabbit mAb were purchased from Cell Signaling Technology (Beverly, Massachusetts, USA), Inc. Phospho-Stat5B Rabbit Antibody was obtained from Abcam CO. (Cambridge, UK).

Techniques: Phospho-proteomics, Control